RAPID COMMUNICATION Interferon-y Gene Expression in Unstimulated Bone Marrow Mononuclear Cells Predicts a Good Response to Cyclosporine Therapy in Aplastic Anemia
نویسندگان
چکیده
Cyclosporine (CyA) therapy has been shown to be effective in some patients with aplastic anemia. In an attempt to characterize aplastic patients likely to benefit from CyA therapy, we examined bone marrow mononuclear cells (BMMC) obtained before therapy from 23 patients with aplastic anemia, who were treated with CyA alone. Expression of four myelosuppressive cytokines, including tumor necrosis factor (TNF), lymphotoxin, macrophage inflammatory protein-la (MIP-la), and interferony (IFN-y) was examined using polymerase chain reaction (PCR)-assisted messenger RNA (mRNA) amplification. mRNAfor TNF, lymphotoxin, and MIP-lawas readily detectable at variable levels in BMMC from normal and transfused controls as well as in BMMC from aplastic patients. In contrast, IFN-y mRNA was only demonstrable in
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